Figure 4

Omnivores Chia activity is higher than that of carnivores and herbivores. (a) The schematic representations of the E. coli-expressed bovine, dog, pig, chicken and mouse Chia fusion proteins. Chia is a secreted protein with a molecular mass of approximately 50 kDa, which contains an N-terminal catalytic domain (CatD) and a C-terminal chitin-binding domain (CBD). The E. coli-produced proteins contain the affinity tail of Protein A at the N-terminus. E. coli-recombinant proteins contain V5-His at the C-terminus (Supplementary Fig. S3). (b) Western blot analysis of the recombinant proteins using anti-V5 antibody. Arrow highlights the positions of the fusion proteins (Protein A-Chia-V5-His). The image of (d) was cropped from original full-length gel image shown in Supplementary Fig. S4a. (c) Comparison of the chitinolytic activities of Chia proteins using 4-NP-chitobioside. Error bars represent the mean ± SD from a single experiment conducted in triplicate. **p < 0.01.