Figure 3
From: Repression of Akt3 gene transcription by the tumor suppressor RIZ1
Akt3 gene promoter ChIP assay. (A) ChIP in transfected cells. HEK293 cells were transfected with pcDNA3.1/RIZ1-Flag or pcDNA3.1 vectors (Negative). PCR was performed using primers encompassing human Akt3 promoter region CpG 166 (chr1:243849411-243851290). (B) ChIP-qPCR assay in transfected cells. Data represent mean ± SD of triplicate measurements. *p < 0.05. (C) ChIP in mouse tissues. Liver tissues from 5 weeks old RIZ1 WT and KO mice were used for ChIP assay. PCR was performed using primers encompassing mouse Akt3 promoter region CpG 146 (chr1:177257300-177259121). (D) ChIP-qPCR assay in mouse livers. Data represent mean ± SD of triplicate measurements. *p < 0.05.
