Figure 4
From: Repression of Akt3 gene transcription by the tumor suppressor RIZ1
Binding to Akt3 promoter by recombinant RIZ1 protein fragments. (A) Shown is a Coomassie Blue stained gel of partially purified GST fusion proteins of RIZ1 containing zinc finger motifs 1–3 and 8 (pGST-RIZ1-Zf1–3 and Zf8). The arrow from left to right pointed to the protein bands of Zf1-3, Zf8 and pGST respectively. (B) EMSA results. EMSA was performed using purified pGST-RIZ1-Zf1-3 and Zf8 fusion proteins and biotin end labeled probes of Akt3 promoter region CpG146. Un-labeled probes (30 fold excess) were used in competition experiments.
