Figure 5
From: Conformational folding and disulfide bonding drive distinct stages of protein structure formation
Schematic illustration of the folding pathway of CRP. The initial folding of strands A and B promotes correct assemblage of strands C to I. This brings Cys36 and Cys97 in close proximity and their bonding is ensued with the coverage by a.a. 168–176 helix. At this stage, strands B and L are misassembled in relation to strands C to I. Subsequently, calcium binding by Asp60, Asn61 on strand E and Gln139 on strand J guides the assemblage of strands J and K with strands C to I, displacing strands B and L to the right location. After the integration of strands J and K, CRP subunit attain a near-native conformation that allows pentamer assembly.
