Table 1 All strains and plasmids used in this study.
From: Isolation and characterization of novel mutations in the pSC101 origin that increase copy number
Strain | Notes | Reference | JBEI ID |
|---|---|---|---|
E. coli DH10B | Â | Invitrogen | Â |
E. coli DP10 | ∆araFGH ∆P-araE pCP18-araE: for linear arabinose induction |  | |
E. coli XL1-Red | Commercial mutator strain | Agilent | Â |
Plasmid | Notes | Reference | Â |
pBbS8k-RFP_R43W | RepA Mutant, Kan | This work | JPUB_009625 |
pBbS8k-RFP_R46W | RepA Mutant, Kan | This work | JPUB_009627 |
pBbS8k-RFP_R46Q | RepA Mutant, Kan | This work | JPUB_009629 |
pBbS8k-RFP_M78I | RepA Mutant, Kan | This work | JPUB_009631 |
pBbS8k-RFP_E83K | RepA Mutant, Kan | This work | JPUB_009633 |
pBbS8k-RFP_E93G | RepA Mutant, Kan | This work | JPUB_009635 |
pBbS8k-RFP_N99D | RepA Mutant, Kan | This work | JPUB_009637 |
pBbS8k-RFP_N99K | RepA Mutant, Kan | This work | JPUB_009639 |
pBbS8k-RFP_K102E | RepA Mutant, Kan | This work | JPUB_009641 |
pBbS8k-RFP_E115K | RepA Mutant, Kan | This work | JPUB_009643 |
pBbS8k-RFP_R43W_E93G | RepA Double Mutant, Kan | This work | JPUB_009645 |
pBbS8k-RFP_K102E_E115K | RepA Double Mutant, Kan | This work | JPUB_009647 |
pBbS8k-RFP | AraC Expression Vector, pSC101 Ori, Kan | Â | |
pBbA8k-RFP | AraC Expression Vector, p15a Ori, Kan | Â | |
pBbE8k-RFP | AraC Expression Vector, ColE1 Ori, Kan | Â | |
pBbE8a-RFP | AraC Expression Vector, ColE1 Ori, Amp | Â | |
pBbB8a-RFP | AraC Expression Vector, BBR Ori, Amp | Â | |
pBbS8a-RFP | AraC Expression Vector, p15a Ori, Amp | Â | |
pMGT1 | ChnR Biosensor with TetA Reporter, pSC101 Ori, Spec | This work | JPUB_009650 |
pBbSlactamC-mCherry (star) | ChnR Biosensor with mCherry Reporter, pSC101 Ori, Spec | Â |
