Figure 6
From: Modulation of oncogenic miRNA biogenesis using functionalized polyamines
Footprinting analyses. (A) Sequence and secondary structure of pre-miR-372; (B) Lead(II) acetate probing of pre-miR-372 in the presence of 0, 1, 5, 10 and 50 µM of compound PA-1 (lanes 4–8). (B) Human Recombinant Dicer probing of pre-miR-372 in the presence of 0, 0.5, 1 and 5 µM of compound PA-1 (lanes 4–7). (C) RNase S1 probing of pre-miR-372 in the presence of 0, 1, 5, 10 and 50 µM of compound PA-1 (lanes 4–8). In all gels, lane 1 represents intact RNA, lane 2 and lane 3 represent the alkaline hydrolysis ladder and T1 digestion ladder, respectively. Gels were cropped for clarity and complete gels are presented in Supporting Information Fig. S9.
