Figure 4
Effect of QFAE on pulmonary inflammation in ALI mice induced by LPS. (A) Effect of QFAE on number of inflammatory cells in BALF from LPS-induced mice (100× ). (a) Blank control group, (b) Drug control group, (c) LPS group, (d) QFAE (25 g/kg) + LPS groups, (e) QFAE (7.5 g/kg) + LPS groups, (f) QFAE (2.5 g/kg) + LPS groups. Scale bar, 100 μm. (B) Effect of QFAE on neutrophils in BALF. (C) Effect of QFAE on macrophages in BALF. (D) Effect of QFAE on lung W/D ratio. (E) Effect of QFAE on expression of MPO in cells from BALF by Western blot. (F) Protein level of MPO was normalized to the level of β-actin, the loading control. Data are presented as the means ± SEM, *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the LPS group, ††P < 0.01 and †††P < 0.001 compared with the blank control group.
