Figure 2
From: Real-time TIRF observation of vinculin recruitment to stretched α-catenin by AFM
AFM-based extension experiment and structural imaging for α-catenin. (a,b) Force versus time curves from the contact phase. Tensile force is shown as positive and five individual force curves are overlaid together. Force curves were classified as (a) those with successful α-catenin extension; and (b) those without α-catenin extension, based on the value of rupture force, FR, as indicated by an arrow. (c,d) Contour maps of number densities of force-time data points in the hold time in each group. Color contours are set from 0.2 times (light) to 0.85 times (dark) of the maximum value of the number density. (e,f) Results of AFM structural imaging. The images show (e) the autoinhibited conformation of the wild-type α-catenin fragment and (f) the opened conformation of the mutated fragment.
