Figure 3
From: Real-time TIRF observation of vinculin recruitment to stretched α-catenin by AFM
Real-time TIRF observation of vinculin recruitment to stretched α-catenin by AFM. (a) Square pulses with increase in intensity after 1.68 s, representing vinculin recruitment to α-catenin under tension and a decrease at 3.68 s, representing vinculin dissociation caused by further unfolding of α-catenin. The time courses of relative intensity were shown in the bottom part of the panel. (b) Step increases in intensity, independent of the timing of AFM experiment, which was caused by nonspecific vinculin interaction with the coverslip.
