Figure 1
From: Operation of a P300-based brain-computer interface in patients with Duchenne muscular dystrophy

A region-based two-step P300-based hiragana speller. The subject was required to count the number of intensifications of the green/blue circle containing the target character to be input. Each circle flashed eight times, and the interval between two flashes was 175 ms, consisting of 100 ms of intensification (green) and 75 ms of rest (blue). We divided the 6 × 9 matrix into six circled regions including nine characters each. First, each region was intensified individually. When a region that included a target character was selected, the speller matrix moved to the second step. The second step used 3 × 3 regions with one character each, and each region was again intensified.