Table 1 Primer sequence for proliferation genes.
Gene symbol | Accession number | Forward Primer (5′-3′) | Reverse Primer (5′-3′) | Product size |
|---|---|---|---|---|
ATM | NM001162400.1 | TTGCCACACTCTTTCCATGT | CCCACTGCATATTCCTCCAT | 110bp |
cdc25 | NM001199572.1 | AGCGAAGATGATGACGGATT | GCAGAGATGAAGAGCCAAAGA | 163bp |
CDK2 | NM_001199857.1 | TCCGTATCTTCCGCACGTTG | GCTTGTTGGGATCGTAGTGC | 276bp |
CDK6 | NM_001007892.2 | CCAGACCCGCACAACCTATT | TCTTGGCTGGATTGAACGCT | 96bp |
cdc2 | NM205314.1 | TCTGCTCTGTATTCCACTCCTG | ATTGTTGGGTGTCCCTAAAGC | 144bp |
Chk2 | NM001080107 | AGACCAAATCACTCGTGGAGAATAC | GATGCTCTAAGGCTTCCTCTATTGT | 140bp |
cyclin D1 | NM_205381.1 | GACTTTTGTGGCTCTGTGCG | CTGTTCTTGGCAGGCTCGTA | 202bp |
cyclin E1 | NM_001031358.1 | CGCCACCACAAAGCAGTAAG | TCACCGGCAGCATTTCCATA | 137bp |
cyclin B3 | NM205239.2 | ATCACCAACGCTCACAAGAAC | AGGCTCCACAGGAACATCTG | 171bp |
p21 | AF513031.1 | TCCCTGCCCTGTACTGTCTAA | GCGTGGGCTCTTCCTATACAT | 123bp |
p53 | NM_205264.1 | TGGAACCATTGCTGGAACCC | AGTTGCTGTGATCCTCAGGG | 127bp |
PCNA | AB053163.1 | GATGTTCCTCTCGTTGTGGAG | CAGTGCAGTTAAGAGCCTTCC | 104bp |
β-actin | L08165 | TGCTGTGTTCCCATCTATCG | TTGGTGACAATACCGTGTTCA | 178bp |
