Figure 5

Curcumin increases intracellular ROS levels to inhibit leukemic cell proliferation. (A) Cells (5 × 105 cells/mL) were treated with curcumin (50 μM), GSH (5 mM), and both for 24 hours, and subjected to the ROS detection analysis using FACS. (B) Cells (3 × 105 cells/mL) were cultured in the presence of curcumin (50 μM), GSH (5 mM), and both, and enumerated every day using the trypan blue staining method. Results are the average of three independent experiments (means ± SD).