Table 1 Polymerase reads sequence statistics.

From: SMRT sequencing of full-length transcriptome of flea beetle Agasicles hygrophila (Selman and Vogt)

Sample name

cDNA size

SMRT cells

Polymerase reads

Post-filter polymerase reads

Post-filter total number of subread bases

Post-filter number of subread

Post-filter subreads N50

Post-filter mean subread length

T01

0.5–1 K

2

300,584

158,893

3,021,283,493

3,163,026

938

955

T01

1–2 K

2

300,584

199,791

4,169,421,605

2,534,817

1,648

1,644

T01

2–6 K

1

150,292

98,310

2,263,026,061

801,048

2,835

2,825

  1. cDNA size: insert fragment size of cDNA libraries; SMRT cells: the number of cells used for library construction; Polymerase reads: the number of polymerase reads sequences after sequencing; Post-filter polymerase reads: the number of polymerase reads sequences after filtration; Post-filter total number of subread bases: the number of subreads bases after filtration; Post-filter number of subread: the number of subreads after filtration; Post-filter subreads N50: subread N50 length after filtration; Post-filter mean subread length: average length of subread after filtration.
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