Table 3 Full-length sequences statistics

From: SMRT sequencing of full-length transcriptome of flea beetle Agasicles hygrophila (Selman and Vogt)

Sample

cDNA size

Reads of insert

Number of five prime reads

Number of three prime reads

Number of poly-A reads

Number of filtered short reads

Number of non-full-length reads

Number of full-length reads

Number of full-length non-chimeric reads

Average full-length non-chimeric read length

Full-length Percentage (FL%)

Artificial concatemers (%)

T01

0.5–1 K

122,928

68,904

78,474

73,850

27,518

40,363

55,047

53,115

688

44.78%

3.51%

T01

1–2 K

142,751

76,467

85,726

83,554

23,630

55,571

63,550

63,051

1,225

44.52%

0.79%

T01

2–6 K

69,366

49,331

50,528

50,045

2,192

25,127

42,047

41,919

2,697

60.62%

0.30%

  1. cDNA size: insert fragment size of cDNA libraries; reads of insert: the number of reads of insert (ROI) sequences; Number of five prime reads: the number of ROI sequences containing 5′ primer; Number of three prime reads: the number of ROI sequences containing 3′ primer; Number of poly-A reads: the number of ROI sequences containing poly-A; Number of filtered short reads: the number of filtered ROI of <300 bp; Number of non-full-length reads: the number of non-full-length ROI; Number of full-length non-chimeric reads: the number of full-length non-chimeric ROI; Average full-length non-chimeric read length: average length of full-length non-chimeric sequence; Full-length percentage (FL%): the percentage of full-length sequence in ROI sequence; Artificial concatemers (%): the percentage of full-length chimeric sequence in full-length sequence.
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