Figure 1 | Scientific Reports

Figure 1

From: miR-429 suppresses tumor migration and invasion by targeting CRKL in hepatocellular carcinoma via inhibiting Raf/MEK/ERK pathway and epithelial-mesenchymal transition

Figure 1

miR-429 translationally regulated CRKL expression by targeting its 3′-UTR selectively. (A) Putative binding sites for miR-429 at CRKL-3′-UTR. (B) Luciferase activity assay of the interaction between miR-429 and CRKL-3′-UTR. HepG2 cells were co-transfected with miR-429 or miR-NC and psiCHECK-2-CRKL-3′-UTR-WT or psiCHECK-2-CRKL-3′-UTR-MUT for 48 h. Renilla luciferase activity was normalized to Firefly luciferase activity. **P < 0.01, ***P < 0.001 refer to the differences were significant compared with negative control. (C,D) miR-429 negatively regulated endogenous CRKL at protein level rather than at mRNA level. The endogenous CRKL protein and mRNA levels were measured by WB and qRT-PCR in HepG2 cells transfected with miR-429, miR-NC, LNA-miR-429, LNA-miR-NC for 48 and 72 h, respectively. ACTB was used as the internal reference. **P < 0.01 refers to difference obtained in comparison with the negative control.

Back to article page