Figure 6

Hepatic (A,C) and renal (B,D) expression levels of lipogenic enzymes measured using rtPCR: fatty acid synthase (FAS) (A,B), acetyl-CoA carboxylase alpha (ACC alpha) (C,D). Measurements were obtained in liver and kidney tissue samples from age and sex matched WT (Fa/Fa) (n = 4), obese Zucker (fa/fa) (n = 9) and WT-STZ (n = 6) rats. The fold changes were measured relative to WT (Fa/Fa) controls and calculated by 2−ΔCt method. The data were normalized to β-actin. All data represent mean + /−S.E.M. from three independent experiments. Statistical analysis was performed by one-way ANOVA with Tukey correction for multiple comparisons. (*p < 0.05, ***p < 0.001).