Figure 4
Quantitative RT-PCR analyses of five key flowering pathway genes (Hd1, Ehd1, Ghd7, Hd3a, and RFT1) in parents and NILs under natural LD and SD conditions. Transcript levels in leaves sampled 55 days after planting were measured in three biological replicates with three technical replications. The mean values of the relative expression levels of genes in BRNIL-20, CRNIL-58, ‘Bengal’, and ‘Cypress’ were compared with ‘PSRR-1’. Standard deviations are indicated by the error bars. The rice Actin1 gene was used as the internal control for normalization.
