Figure 4
Nodulation phenotype of MtNRLK1 RNAi or overexpressing roots and of an insertional mutant plant. (A,B) Nodulation phenotype of roots expressing a RNAi construct to silence MtNRLK1. (A) Analysis of MtNRLK1 expression by real-time RT-PCR in roots expressing a GUS RNAi (control) or a MtNRLK1 RNAi construct (#1 or #2). Error bars represent Standard Deviation (SD; n > 4 plants/construct). (B) Nodule number in composite plants expressing a GUS RNAi or a MtNRLK1 RNAi construct at 7 and 14 days post-inoculation (dpi). Two independent biological experiments are shown and error bars represent confidence intervals (α = 0.05; n > 30). Letters indicate statistically significant differences (Kruskal-Wallis test, α < 0.05). (C) Nodule number in nrlk1 mutant plants was compared to the Wild-Type (WT) 14 days post-inoculation (dpi). Two biological replicates are shown and error bars represent confidence intervals (α = 0.05) (n > 20). Letters indicate significant differences (Mann & Whitney test, α < 0.05). (D,E) Nodulation phenotype of roots overexpressing MtNRLK1. (D) Analysis of MtNRLK1 expression by real-time RT-PCR in roots overexpressing GUS (35S:GUS) or MtNRLK1 (35S:MtNRLK1). Error bars represent SD (n > 4). (E) Nodule number in 35S:GUS or 35S:MtNRLK1 composite plants at 7 and 14 days post-inoculation (dpi). Error bars represent confidence intervals (α = 0.05; n > 23). Letters indicate statistically significant differences (Kruskal-Wallis test, α < 0.05). (F) Nodulation phenotype of nrlk1 roots overexpressing GUS or MtCLE13 at 14 dpi. Two independent experiments are shown and error bars represent confidence intervals (α = 0.05; n > 11). Letters indicate statistically significant differences (Mann & Whitney test, α < 0.05).
