Figure 3
From: Formation of Multi-Component Extracellular Matrix Protein Fibers
Confocal images of composite ECM fibers formed via binding sites. (a–c) Composite FN/COL fibers formed by sequential incubation on 33% PSS micro-patterns: (a) Raw and (b) z-projected confocal images of COL (green) on PSS-induced FN fibers (red), and (c) fluorescence intensity profile from (b) along the z direction. (d–f) Composite FN/COL fibers formed by simultaneous incubation on 33% PSS micro-patterns: (d) Raw and (e) z-projected confocal images of mixed COL (green) and FN (red) fibers, resulting in FN (red)-induced COL (green) fibers, and (f) fluorescence intensity profile from (e) along the z direction. (g–i) Sequential incubation of ELAS on FN fibers as a negative control: (g) Raw and (h) z-projected confocal images of ELAS (red) on pre-existing FN fibers (green), and (i) fluorescence intensity profile from (h) along the z direction. The scales of (a,d), and (g) are 50 µm.
