Table 3 T0 maize plants expressing D. v. virgifera hpRNA show robust root protection compared to negative controls. The root damage rating data estimated using 0–1.0 NIS scale was converted into binomial categorical data and the proportion of bioassay passers ( ≤ 0.5 root rating) in each constructs was compared against controls and other constructs were using SAS PROC GLIMMIX procedure (SAS 9.3, 2013).

From: Gene silencing in Tribolium castaneum as a tool for the targeted identification of candidate RNAi targets in crop pests

maize genotype

Construct

# events tested

proportion of plants “passed”**

SEM

DAS59122–7

positive control

30

1a

0

hpRpII140 v1

pDAB114524

21

0.94b

0.05

hpRpII140 v2

pDAB114525

16

0.8b

0.14

hpRop-2 v3

pDAB115770

17

0.73b

0.17

hpdre4–1 v1

pDAB114546

11

0.46bc

0.26

hpdre4–1 v2

pDAB114547

11

0.46bc

0.26

hpncm-1 v2

pDAB119706

6

0d

0

YFP protein

pDAB101556

33*

0d

0

hpYFP

pDAB110853

33*

0.09c

0.06

Non-transformed B104

negative control

34

0d

0

Non-transgenic isoline 7SH382

negative control

32

0d

0

  1. *Transgenic negative control events expressing either yfp hairpin dsRNA or YFP protein.
  2. **Proportion values with the same letter are not significantly different.
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