Figure 6
From: miR-539 acts as a tumor suppressor by targeting epidermal growth factor receptor in breast cancer
EGFR is a direct target gene of miR-539. (A) Bioinformatics analysis of the interaction between miR-539 and its binding site within the 3′-UTR of EGFR. (B) Relative luciferase activity in different groups after co-transfection with psiCHECK-2-EGFR (WT) or (MUT) and miR-539 mimics or mimic control. (C) The introduction of miR-539 reduced the endogenous EGFR mRNA and protein levels in MDA-MB-231 and MCF7 cells. UTR: untranslated region; EGFR: epidermal growth factor receptor; WT: wild type; and MUT: mutant. *P < 0.05.
