Figure 5

The effect of TSH on glucose uptake in INS-1 cells. After 6 h starvation, INS-1 cells were incubated with DMSO or an inhibitor of p38 MAPK (SB203580) for 30 min, and following treated by TSH at 100 µIU/ml for 24 h. Then the glucose uptake (green) was checked as per the manufacturer’s instruction. NC-Pal served as the negative control and cells treated with DMSO were used as a positive control. The magnification of third and fourth rows are 400 times while the magnification of first and second rows are 200 times. Bar = 40 µm.