Figure 5
From: IGFBPL1 Regulates Axon Growth through IGF-1-mediated Signaling Cascades
Expression of IGF-1 and IGF-1R in the developing retina. (A) Epifluorescence photomicrographs of retinal sections taken from E16, P0, P10 and adult mice double-immunolabeled with primary antibodies against IGF-1 (red; left panel) and Tuj1 (green; middle panel) and counterstained with nuclear marker DAPI (blue). GCL, ganglion cell layer. Scale bars: 50 µm. (B,C) Western blot quantification of IGF-1 (B) and IGF-1R (C) expression in retinal lysates taken from E16, P0, P10 and adult mice. Western blot quantifications were normalized to GAPDH and reported as relative intensity compared to GAPDH in the bar charts.
