Figure 2 | Scientific Reports

Figure 2

From: Exosome-Mimetic Nanovesicles from Hepatocytes promote hepatocyte proliferation in vitro and liver regeneration in vivo

Figure 2

Characteristics of exosome-mimetic NVs produced from primary hepatocytes. (A) Representative TEM images of exosome-mimetic NVs. Scale bars: 200 and 50 nm. (B) (C) Size and zeta potential distribution of exosome-mimetic NVs and exosomes determined by a Zetasizer Nano ZS90 instrument. (D) Exosomal markers (CD9, CD63) on exosome-mimetic NVs were measured with western blotting. (E) Sphk2, which plays an important role in liver regeneration, in exosome-mimetic NVs and exosomes from hepatocytes was measured with western blotting. (F) The yield of exosome-mimetic NVs and exosomes from the same number of primary hepatocytes (5 × 106 cells) was evaluated based on total protein in exosome-mimetic NVs and exosomes using a BCA protein assay. ***P < 0.001. Uncropped western blots are shown in Supplementary Figure 1.

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