Figure 5
From: Heterologous pathway assembly reveals molecular steps of fungal terreic acid biosynthesis
Introduction of atG into strain GS-NXAE improved the biosynthesis of compounds 6 and 7. Three resulting strains (GS-NXAE-G #18, #33, and #36) were selected randomly and tested. Gene copies of atG in each strain were not determined, and the production levels of the two compounds differed among the three GS-NXAE-G strains. Compound 6 was quantified properly, but compound 7 was quantified only as the relative HPLC peak area (the highest titer of GS-NXAE-G #18 after 48 h induction was set to 100%). One-way analysis of variance (ANOVA) was employed to determine significant production differences of compounds 6 and 7 between GS-NXAE and each GS-NXAE-G strain. The P-value was used to check the significance, and it was significant at P < 0.05. OriginPro 8.0 (OriginLab Corporation, USA) was used for ANOVA. **P < 0.01 at 24 h; ##P < 0.01 at 48 h. Detailed P-value for each run was shown in Supplementary Table S8.
