Figure 6
From: Heterologous pathway assembly reveals molecular steps of fungal terreic acid biosynthesis
Biosynthetic molecular steps of terreic acid (TA) that were identified by heterologous pathway assembly. Speculated transition compounds are boxed. The AtX (polyketide synthase) catalyzes formation of polyketide 6-MSA (2); AtA (decarboxylase) catalyzes formation of 3-methylcatechol (5) by decarboxylative hydroxylation of 6-MSA; AtG (cytochrome P450 monooxygenase) helps AtE (cytochrome P450 monooxygenase) to catalyze formation of 3-methyl-1,2,4-benzenetriol (6); AtD (cyclooxygenase) catalyzes formation of terremutin (3) by epoxidation and hydroxyl oxidation of 3-methyl-1,2,4-benzenetriol (6); AtC (GMC oxidoreductase) catalyzes formation of the final product, TA (1), via a reaction of oxidation of the hydroxyl group in terremutin (3).
