Figure 2
From: Ventromedial prefrontal cortex mediates sex differences in persistent cognitive drive for food
Chemogenetic vmPFC silencing in male rats during renewal of conditioned responding to a food cue. The extent of AAV expression within the vmPFC is shown on a modified atlas template (Swanson 2004) (a) and a representative image (b). Experimental design (c). Conditioned responding in the acquisition context (d) and the extinction context (e) and as a difference in responding between the two contexts (f), Representative image of AAV-positive (green), Fos-positive (magenta), and double-labeled (arrow) neurons (g). Total number of AAV-positive (h) or Fos-positive (i) neurons. Percentage of Fos-positive neurons that were double labeled (j). DREADD + CNO group (n = 7 behavior, n = 4 Fos) in red, DREADD + vehicle (n = 6 behavior, n = 4 Fos) in black, Control Virus + CNO (n = 5 behavior, n = 3 Fos) in gray. Box plots show min, 25th percentile, median, 75th percentile and max. Scatterplots show individual data points (dots) with median and inner quartiles (line and error bars). *p < 0.05, **p < 0.01, ***p < 0.001.
