Figure 2 | Scientific Reports

Figure 2

From: Three-dimensional imaging of KNDy neurons in the mammalian brain using optical tissue clearing and multiple-label immunocytochemistry

Figure 2

Imaging of the intact KNDy neuron population following immunolabelling and optical tissue clearing. (A) Rat brain block before (i) and after (ii) immunolabelling and clearing. (B) Projection (optical thickness = 200 µm) of kisspeptin (red) and neurokinin B (green) immunolabelling imaged through the sagittal plane of the arcuate nucleus of ovariectomized rats implanted with either sesame oil vehicle (OVX + VEH, (B)) or estradiol (OVX + E2, (C)). R = rostral, D = dorsal.

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