Figure 3 | Scientific Reports

Figure 3

From: Three-dimensional imaging of KNDy neurons in the mammalian brain using optical tissue clearing and multiple-label immunocytochemistry

Figure 3

Kisspeptin and neurokinin B immunoreactivity in the cleared rat brain. (A) Single optical slices imaged in the horizontal plane with imaging depths of 150 µm (a), 300 µm (b), 450 µm (c) and 750 µm (d) from the ventral surface of the brain. (a–c) ARC kisspeptin and neurokinin B cell bodies. (d) Kisspeptin and NKB fibers in the dorsomedial hypothalamus (DMH). The z-depth of a-d corresponds with the dotted lines in image Fig. 2 (C). (i-iii) High magnification images of kisspeptin (i), neurokinin B (ii) and combined immunolabelling (iii) corresponding with insets from (a–d). (iv) Reduced colour saturation reveals dense KNDy fiber projections along the wall of the third ventricle at 450 µm depth from the ventral surface of the ARC. (B) Images projected into the coronal plane using IMARIS software showing kisspeptin and neurokinin B immunoreactivity in the rostral to caudal ARC. R = rostral, C = caudal.

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