Figure 6 | Scientific Reports

Figure 6

From: Three-dimensional imaging of KNDy neurons in the mammalian brain using optical tissue clearing and multiple-label immunocytochemistry

Figure 6

Immunolabelling and optical tissue clearing in the ovine hypothalamus. (A) Ovine hypothalamic block before (i) and after immunolabelling and optical tissue clearing as viewed in the horizontal (ii) and sagittal (iii) plane. (B) Projected image of kisspeptin neurons in the ovine arcuate nucleus (ARC) in the sagittal plane (optical thickness = 250 µm). Dotted outline represents the dorsal border of the ARC. (C–F) Single optical slices (4 µm optical thickness) through the ARC imaged in the horizontal plane. The z-depth of each image corresponds with lines (C–F) in image (B). (C–F) (i) High magnification images of kisspeptin neurons corresponding with the insets in C-F. D = dorsal, V = ventral, R = rostral, C = caudal.

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