Table 1 Biomass composition and growth parameters of Methylomicrobium alcaliphilum 20Z. *CO₂ production rates are below expected due to high pH of the growth medium.

Amino Acids

43

2

Alanine

0.366

M. alcaliphilum 20Z

This study

Arginine

0.149

-“-

-“-

Asparagine

0.119

-“-

-“-

Aspartate

0.409

-“-

-“-

Cysteine

0.024

-“-

-“-

Glutamate

0.487

-“-

-“-

Glutamine

0.15

-“-

-“-

Histidine

0.078

-“-

-“-

Glycine

0.339

-“-

-“-

Isoleucine

0.203

-“-

-“-

Leucine

0.332

-“-

-“-

Lysine

0.182

-“-

-“-

Methionine

0.093

-“-

-“-

Phenylalanine

0.171

-“-

-“-

Proline

0.161

-“-

-“-

Serine

0.194

-“-

-“-

Threonine

0.229

-“-

-“-

Tryptophan

0.072

-“-

-“-

Tyrosine

0.111

-“-

-“-

Valine

0.278

-“-

-“-

Ectoine

1.5

0.1

0.106

-“-

-“-

Lipids

14

1

C14 (myristate)

0.010

M. alcaliphilum 20Z

-“-

C15 (pentadecylic acid)

0.002

-“-

-“-

C16 (palmitate)

0.329

-“-

-“-

C18 (stearate)

0.002

-“-

-“-

  Phosphatidylserine (1%)*

0.002

-“-

²⁶

  Phosphatidylethanolamine (50%)*

0.086

-“-

-“-

  Dipalmitoyl phosphatidate (4)*

0.007

-“-

-“-

  Cardiolipin (11)*

0.019

-“-

-“-

  Phosphatidylglycerol (34)*

0.059

-“-

-“-

Sterols and bacteriohopanepolyol

0.02

tetrahymanol

0.025

M. alcaliphilum 20Z

⁵⁹

aminotriol (III), 65% of BHP

0.008

-“-

⁶⁰

3-Me-aminotriol, 31% BHP

0.003

-“-

-“-

aminotetrol and 3-Me-aminotetrol, <3% BHP

-“-

-“-

0.001

Intracellular metabolites

1.5

—

  Ribulose-5-phosphate/Ribose-5-phosphate

0.001

M.aclaliphilum 20Z

16

Fructose-1, 6-bisphosphate

0.001

-“-

-“-

Fructose-6-phosphate

0.003

-“-

-“-

Glucose-6-phosphate

0.002

-“-

-“-

  Glyceraldehyde-3-phosphate/Dihydroxyacetone

0.003

-“-

-“-

6-Phosphogluconic acid

0.00015

-“-

-“-

2-dehydro-3-deoxy-phosphogluconate

0.000003

-“-

-“-

Phosphoglycerate

0.006

-“-

-“-

Phosphoenolpyruvate

0.005

-“-

-“-

Pyruvate

0.015

-“-

-“-

Acetyl-CoA

0.0001

-“-

-“-

Succinate

0.002

-“-

-“-

Malate

0.004

-“-

-“-

Fumarate

0.001

-“-

-“-

Citrate

0.001

-“-

-“-

Glycerate

0.001

-“-

-“-

  ATP

0.005

Methylomonas methanica

⁶¹

  ADP

0.002

M. extorquens AM1

⁶²

  AMP

0.001

M. extorquens AM1

-“-

  NAD

0.002

M. extorquens AM1

-“-

  NADH

0.002

M. extorquens AM1

-“-

  NADP

0.001

M. extorquens AM1

-“-

  NADPH

0.001

M. extorquens AM1

-“-

  polyP (PPi)

0.029

Methylomonas methanica

⁶¹

Cytochrome c

0.00036

M. alcaliphilum 20Z

³⁷

B12

0.000000063

-“-

⁶³

Ubiquinol-8

0.00022

Assumption

⁶²

Protoheme

0.00022

Assumption

-“-

coenzyme-A

0.00022

Assumption

-“-

FMN

0.00022

Assumption

-“-

FMNH2

0.00022

Assumption

-“-

FAD

0.00022

Assumption

-“-

SAM

0.00022

Assumption

-“-

Glutathione

0.00022

Assumption

-“-

Carbohydrates

1.38

0.2

Mannose

0.002

M. alcaliphilum 20Z

⁶⁴

Ramnose

0.0002

-“-

-“-

Glycogen

8.1

1.4

0.448

-“-

This study

Sucrose

0.9

0.2

0.026

-“-

⁶⁵

Ribose

0.036

-“-

⁶⁴

Maltose

0.008

-“-

-“-

Arabinose

0.022

-“-

-“-

Galactose

0.005

-“-

-“-

Cell wall

Peptidoglycan

9.1

-

0.053

Assumed as in M. buryatense 5GB1

²⁴

LPS (lipid IVA and KDO)

0.027

-

0.002

-“-

-“-

RNA

9.7

3.6

This study

ATP

0.050

M. alcaliphilum 20Z

-“-

UTP

0.050

-“-

-“-

CTP

0.047

-“-

-“-

GTP

0.047

-“-

-“-

DNA

4.0

0.28

This study

dATP

0.0021

M. alcaliphilum 20Z

-“-

dTTP

0.0021

-“-

-“-

dCTP

0.0020

-“-

-“-

dGTP

0.0020

-“-

-“-

Ash

5

Assumed as in M. buryatense 5GB1

⁴⁰

BIOMASS (measured)

98.2

8.8

3-PG (balance)

1.8

0.010

TOTAL

100.0

ELEMENTAL COMPOSITION

C

44.9

2.6

M. alcaliphilum 20Z

This study

N

8.1

2.4

-“-

-“-

H

6.8

0.1

-“-

-“-

EXCREATED PRODUCTS

-“-

Formate

0.7

M. alcaliphilum 20Z

-“-

Acetate

0.02

-“-

-“-

Lactate

0.004

-“-

-“-

EPS

0.5–1%

1%

-“-

-“-

DCW (% WCW)

25 ± 3.4

Methane Uptake (mmol g CDW⁻¹ h⁻¹)

11.7 ± 0.14

This study

Oxygen Uptake (mmol g CDW⁻¹ h⁻¹)

14.7 ± 0.9

-“-

O₂/CH₄ Uptake Ratio

1.25 ± 0.06

-“-

CO₂ production (mmol g CDW⁻¹ h⁻¹)*

0.37 ± 0.05

-“-

Specific Growth Rate (h⁻¹)

0.12 ± 0.01

-“-

Methane Uptake (mmol g CDW⁻¹ h⁻¹)

18.6 ± 1.4

This study

Oxygen Uptake (mmol g CDW⁻¹ h⁻¹)

24.4 ± 2.1

-“-

O₂:CH₄ Uptake Ratio

1.31 ± 0.07

-“-

Specific Growth Rate (h⁻¹)

0.14 ± 0.02

-“-