Table 1 Top 50 consensus Z-scores for single mutants.

From: Application of Assisted Design of Antibody and Protein Therapeutics (ADAPT) improves efficacy of a Clostridium difficile toxin A single-domain antibody

Residue

R

K

Q

N

S

T

H

W

Y

F

M

L

I

V

A

G

E

D

S53

−0.9

                 

T54

−1.6

      

−1.4

−1.1

−1.1

        

T56

−1.7

           

−1.2

     

S57

   

−1.0

              

Y59

           

−1.5

      

Q101

    

−0.9

             

T103

−1.5

−1.5

−1.0

    

−1.6

          

R104

  

−1.0

−1.3

   

−5.2

−3.3

−3.4

 

−0.9

      

Q106

       

−1.9

 

−1.2

 

−1.4

−0.9

   

−0.9

 

D107

−1.5

−1.1

−1.3

−1.1

−1.1

−1.1

−1.2

−1.4

−1.5

−1.5

−1.2

−1.2

−1.2

−1.2

−1.1

−1.1

−1.3

 

P108

−1.0

−1.1

     

−1.0

−1.0

−1.0

−1.0

   

−0.9

   

E110

  

−1.0

               
  1. Structure preparation 2 was used, which includes modeled N-terminal residue Q1 of VHH and C-terminal residue G262 of TcdA. Single-point mutations experimentally tested are highlighted in bold. Parent antibody Z-score = −0.33.
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