Figure 4
CD134 is upregulated on human NK cells in the presence of activated T cells. (A) PBMCs or purified NK cells were not treated, co-cultured with Raji cells, or treated with anti-CD3 (0.5 μg/mL) and anti-CD28 (1 μg/mL), or SEB (1 ng/mL) to stimulate T cells for 24 hours. CD134 expression was then examined by flow cytometry. CD134 expression on gated NK cells are shown. Plots representative of triplicate experiments. (B) PBMCs were unstimulated, stimulated with rituximab-treated B-cell targets, or stimulated and co-cultured in the same well with purified NK cells in a transwell plate. Co-cultures were harvested 24 hours later and CD134 expression on NK cells examined. n = 3 independent experiments, **P < 0.01, paired t test. (C) PBMCs or purified NK cells were unstimulated or treated with IL-2 (25 ng/mL) or IL-15 (10 ng/mL) for 24 hours. Cells were then harvested and analysed by flow cytometry. Representative dot plots shown from triplicate experiments.
