Figure 5
From: Ouabagenin is a naturally occurring LXR ligand without causing hepatic steatosis as a side effect
OBG-repressive effects for ENACs were conserved in human embryonic kidney 293 T cells. (A) OBG repressed the mRNA expressions of ENACs. The concentration of ligand was 10−6 M. mRNA expression levels of ENAC-α, ENAC-β, ENAC-γ and ENAC-δ in 293 T cells were determined as described in Fig. 2A. The amount of mRNA was normalised against GAPDH, then compared with vehicle. Bar graphs represent means ± SD, and values followed by different letters are statistically different according to ANOVA followed by SNK tests (P < 0.01).(B andC) LXRβ but not LXRα mediates the regulation of ENACs by OBG. Under LXRα (B) or LXRβ (C) knockdown conditions, mRNA levels of each ENAC subunit in 293 T cells treated with 10−6 M ligand were analysed as described in Fig. 5A.
