Figure 2

Effects of dietary AgNPs on developing larvae. (a) Stacked bar chart showing the relative number of viable and dead larvae on the 4^th day post-exposure to 0, 10, 20, 30, 40 and 50 μg/ml of AgNPs. Each group contained 100 larvae in total. (b) Stereomicroscopic image of the external morphological appearance of surviving larvae on the 4^th day post-exposure. (c) Stacked bar chart demonstrating the distribution of the body-length (BL) heterogeneity among the surviving larvae (i.e., relative percentage of viable larvae (derived from data shown in Fig. 2a) with respect to the range of body lengths) after 4 days of exposure. (d) Cumulative percentage of the surviving larvae (relative to the final pupal number) capable of undergoing pupation between the 4^th and 8^th day after hatching. (e) Percentage of surviving larvae that eventually turned into pupae (relative to the number of larvae in each group). For the experiments shown in Figs 2d and e, each vial was loaded with 100 larvae, and each group contained 300 larvae in total. **(P < 0.01) and ***(P < 0.001) denote significant differences between the control group and exposure groups.