Figure 2 | Scientific Reports

Figure 2

From: Contractile Skeletal Muscle Cells Cultured with a Conducting Soft Wire for Effective, Selective Stimulation

Figure 2

(a) Photograph of the PEDOT/PU wire held by tweezers. The double-headed blue arrow indicates the conductive area. (b) SEM image of the magnified view of the conductive area of the PEDOT/PU wire. (c,d) Phase contrast photomicrographs of the PEDOT/PU wire placed in the PDMS chamber with the myoblasts/hydrogel composite before (c) and after (d) 10 days of culture. (e) Photograph of the myotube-cultured PEDOT/PU wire lifted out from the PDMS chamber. The double-headed white arrow indicates the myotubes. (f,g) Phase-contract (f) and the corresponding fluorescent images (g) of the myotubes cultured around the PEDOT/PU wire. (h) The lower half of the cross-section of a Z-stack fluorescent image of (g). (i) Contractile displacement of the myotubes stimulated with an internal PEDOT/PU electrode wire against different frequency of the applied constant current pulses (5 mA amplitude, 10 ms duration, 50 μC charge injection).

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