Figure 3

EpoB i.p. injection before injury does not influence the corneal wound healing process. (A) The corneal epithelial wounds were stained with sodium fluorescein at the specified time points. (B) The percentage of the wound open at each time point was calculated as shown in the line chart (n = 6 mice at each time point). (C) The number of neutrophils in Zone 4 (Fig. 11) was counted at the specified time point after wounding in the EpoB, vehicle, and normal control groups. (D) Corneas were stained with anti-Ly6g-1-FITC (original magnification: 400X). No significant differences were found compared to the vehicle and normal groups. The results are presented as the mean ± SD. (E) Corneas were triple-stained with FITC-tagged anti-α-tubulin (green), rhodamine-phalloidin (red), and DAPI (blue; original magnification: 400X). The arrows in Fig. 5E indicate dividing cells with their mitotic spindles stained with FITC-conjugated anti-α-tubulin (green). (F) The number of dividing cells was counted at the specified time point after wounding in the EpoB, vehicle, and normal control groups. (G) The total number of dividing cells was counted from 0 h to 48 h after wounding in the EpoB, vehicle, and normal control groups. No significant differences were found compared to the vehicle and normal groups. The results are presented as the mean ± SD. A factorial design ANOVA was performed to analyze the differences between the two groups, and Student’s t-test was used to compare the replicates by time point. *P < 0.05, **P < 0.01, ***P < 0.001; n = 6 corneas/group.