Figure 3

Mass spectrometric analysis over time during the 13C-labelling experiments. Sodium 13C-bicarbonate (final concentration, 4 mM) was added on day 0 of the labelling experiments. The 13C-labelling patterns of K37:2 alkenone in cells grown at 25 °C are shown for days 0, 3 and 4. The 13C-labelling patterns in cells grown at 15 °C on day 4 (24 h after the change to 15 °C temperature) are shown for K37:2 and the newly formed K37:3. See Fig. 2 for growth curves and changes in alkenone contents.