Figure 2
Correlation of the IgA-based ELISA with q-Western blotting. (a) A control sample representing 100 ng of recombinant RBP4 (lane 1) and five human serum samples (1 μl each, lanes 2 through 6) were subjected to Western blotting using AG102 as a detector antibody. (b) Five serum samples from normal donors and the recombinant RBP4 protein were tested simultaneously with the standard, and the band intensity values from Western blot images of (a) were interpolated to determine RBP4 quantification. Using a phosphor-image analyser, the reactive area (denoted by AU/mm2) was calculated and plotted with the RBP4 concentrations determined by the IgA-based ELISA. (c) Quantification results for both methods. Coefficient of determination (R2) is indicated.
