Figure 6

NTS inhibits ER stress and UPR activation as it inhibits 3T3-L1 adipocyte differentiation. (A) Protein expression profiles for ER stress-related protein and UPR related protein. Cell lysates were harvested at the indicated time points after the start of adipocyte differentiation and analyzed by Western analysis. Each Western-blotting band represents three experiments performed in triplicate. (B) Immunocytochemical assay for CHOP (ER stress marker, red) and DAPI (nucleus stain, blue). Scale bar = 100 μm.