Figure 3
From: Accelerated invagination of vacuoles as a stress response in chronically heat-stressed yeasts
Changes in the vacuolar morphology under chronic heat stress in various mutant cells. (a) Vph1-GFP fluorescence of wild-type (ScHY-2445), ubi4Δ, and vps23Δ cells at 25 °C and 40.5 °C for 6 h. (b) FM4-64 staining of vps23Δ, vps24Δ, pep12Δ, atg1Δ, atg9Δ and ivy1Δ cells at 25 °C and 40.5 °C for 6 h. The contrast of images of cells at 25 °C was enhanced. Images for the same contrasts at 25 °C and 40.5 °C are shown in Supplementary Fig. 2. Arrows and arrow heads indicate vesicle-like structures and highly movable FM-4-64 fluorescent materials, respectively. (c) GFP and FM4-64 fluorescence of cells expressing Rpl24A-GFP at 40.5 °C for 6 h in control and pep4Δ cells. Arrows indicate vesicle-like structures.
