Figure 5

Real-time PCR detection of host genes and western blot detection of host proteins in HIV-1 and HIV-2 infected MDMs Day-7 post infection. (A) Real-time PCR data shows results from 3 independent experiments. Values are fold-change plotted on a Log scale for HIV-1 (black) and HIV-2 (red) infected MDMs relative to an uninfected control MDMs. Each qPCR amplification was normalized with endogenous GAPDH control and measured the relative amount of target gene. Expression of fold change is calculated as n-fold difference in expression of gene-of-interest relative to GAPDH gene for infected and uninfected cells as n-fold = 2^ − (ΔCt infected −ΔCt uninfected). Each sample was run in triplicate to ensure accurate fold change estimation and the results expressed as mean ± SEM. (B) Western blot data depicts results from proteins isolated from a single donor. Images of the western blots presented here are displayed in cropped format. Full length blots are presented in Supplementary Figure 4. Separate protein gels were run under the same experimental condition and processed in parallel. β-actin was used as a loading control. Western blot data is representative of 3 independent experiments with MDMs derived from 3 independent donors.