Figure 4 | Scientific Reports

Figure 4

From: Identification of novel cell-impermeant fluorescent substrates for testing the function and drug interaction of Organic Anion-Transporting Polypeptides, OATP1B1/1B3 and 2B1

Figure 4

Kinetics of uptake of CB, AF405 and Fl-MTX in A431 cells overexpressing OATP1B1, 1B3 or 2B1. Transport was measured in 96-well plates. Cells were incubated with increasing concentrations of Fl-MTX, CB or AF405 in the linear phase of uptake (2.5 minutes for Fl-MTX, 10 minutes (1B1, 2B1) or 15 minutes (1B3) for CB, and 15 minutes for AF405). Transport capacity was calculated based on calibration with known amounts of the dye. Uptake in A431-OATP cells without background signal (fluorescence in A431-mock cells) is shown. Data points indicate average ± SD values obtained in three independent experiments.

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