Figure 1 | Scientific Reports

Figure 1

From: Impaired Wnt signaling in dopamine containing neurons is associated with pathogenesis in a rotenone triggered Drosophila Parkinson’s disease model

Figure 1

Isolation of dopamine producing cells following rotenone treatment. Brains of adult animals of the genotype TH-GAL4 > 20xUAS-mCD8::GFP from control animals (A,A′) as well as from animals treated for 10 d with rotenone (B,B′) were analyzed using anti GFP immunohistochemistry. GFP-positive, dopamine containing neurons are shown (arrows) in two different Z-areas (A,A′,B,B′). (C) Exposure of rotenone for different periods induced climbing disability in a time dependent manner. The distance reached by animals climbing a vertical plane within 20 s is given. Shown are mean values of three independent experiments, ±SEM, n = 20 individuals for each group (**P < 0.01, ***p < 0.005). (D,E) Magnetic bead assisted isolation of dopamine containing cells using beads coated with anti-mCD8 antibodies. (D) Cell suspension of decollated neurons containing GFP-positive (green arrows) as well as non-labeled neurons (white arrows). (E) GFP-positive cells (green arrows) and magnetic beads (white arrow heads) after bead-assisted isolation devoid of non-labeled cells.

Back to article page