Figure 6
Dose-dependent recovery from intoxication. Vero-d2EGFP cells were incubated with various concentrations of (a) ricin, (b) Stx1, (c) EtxA, or (d) Dtx for 20 h and then chased for 48 h in the absence of toxin. Left columns: Orange, blue, and red lines were derived from cytofluorometry analysis of toxin-treated Vero-d2EGFP cells at the end of the 20 h toxin incubation or the end of the 48 h chase. Unintoxicated parental Vero cells (black lines) and unintoxicated Vero-d2EGFP cells (green lines) were also processed for each condition. Right columns: At the end of the 48 h chase, representative images of cells initially exposed to the stated toxin concentrations were taken at 200× magnification. Cell viability, as assessed by MTS assay (n = 3, avg. ± std. dev.) or annexin V (AV) / 7-AAD staining of the same cell population processed for EGFP cytofluorometry, is indicated. These experiments were performed at the same time as the data presented in Fig. 5.
