Figure 5

pH dependence of the fluorescence of DND particles in water. (A) Fluorescence spectra for NP solutions of pH 11.8 to pH 3.7. Excitation wavelength is 450 nm in all cases. (B) Normalized fluorescence intensity as a function of pH. (C) Spectral fluorescence peak position. (D) Long fluorescence lifetime component (τ2). See SI Figure S6 and S7 for fluorescence decay raw data and analysis. Yellow regions indicate the pKa range of carboxylic acids.