Figure 3

Requirement of CD42b in dengue virus infection. MEG-01 cells were infected with DENV (MOI = 0.5) for 2 hours and washed with PBS. The cultures were maintained in fresh medium for another 7 days before being triple immunostained. Flow cytometry standard data files were analyzed in three-dimension using custom-written software in MATLAB. All the fluorescence intensities were shown as log10 of the actual data. (A) Representative three-dimensional plot of surface CD42b, surface CD41 and intracellular DENV. (B) The relative DENV infection in CD42b⁻CD41^−/+ and CD42b⁺CD41^−/+ subpopulation (n = 4). (C) The relative DENV infection in CD42b⁻CD41⁺, CD42b⁻CD41⁻, CD42b⁺CD41⁺ and CD42b⁺CD41⁻ subpopulation (n = 4). Error bars represent mean ± SD. Statistical significance was determined by using the two-tailed Mann–Whitney test. (D) Proposed model for the requirement of CD42b in dengue virus infection.