Figure 5

Dengue virus replicates itself in CD42b⁺ cells and eventually kills those cells. MEG-01 cells were infected with DENV (MOI = 0.5) for 2 hours and washed with PBS. The cultures were maintained in fresh medium before performing RT-PCR and apoptosis assays. (A) RT-PCR of the culture medium of DENV-infected Vero cells, the culture media of uninfected and DENV-infected MEG-01 cells. dpi = days post infection. Infected-1 and -2 experiments were 2 independent experiments. (B) Apoptosis assays of uninfected and DENV-infected MEG-01 cells at 7 dpi. Early apoptosis means Annexin V⁺ PI⁻ cells and late apoptosis means Annexin V⁺ PI⁺ cells. Data are presented as mean ± SEM of four independent experiments. The Mann-Whitney U test was used to assess the significance of differences between the observed data. *p < 0.05.