Figure 6
Overlay of two separate two-dimensional gels of soybean nodule proteins using Delta2D software. Nodule cytosolic proteins were separated by isoelectric focusing (pI 4–7) followed by second dimension SDS-PAGE on a 15% gel. Resolved proteins were visualized by staining the gel with colloidal Coomassie Blue G-250. Gels were scanned and resulting images were assigned two different colors (green = non-transformed wild-type nodules; red = OASS overexpressing transgenic mutant nodules) and overlaid using Delta2D software in order tvisualize the differences between the two. Yellow demonstrates similar protein quantities in each. Red color indicates a much lower abundance of that particular protein in the non-transformed wild-type nodules. MALDI-TOF-MS identified the 34 kDa protein spot (A) as OASS and the 22 kDa protein spot (B) as phosphinothricin acetyltransferase. Abundant leghemoglobulin protein spots are circled.
