Figure 4

Quantification of liver responses in ERE-Kaede-Casper transgenic zebrafish exposed to EE2 at different stages of development, as determined by fluorescence induction. Responses in the liver were quantified after EE2 exposure at 3–5 dpf, 5–7 dpf and 9–11 dpf. Quantification of liver responses in the E-Chemical treatment groups were normalized against their respective controls. Data are reported as mean fold induction ± SEM (n = 18). Statistical significance values were calculated using ANOVA and Games-Howell post-hoc test (*p < 0.05 and **p < 0.01).